Elevating Microscopy: The Critical Role of Mounting Media in Preserving Precision and Clarity. New cooperation with ADVI
Mounting media, often regarded as the unsung heroes of microscopy, play a pivotal role in the world of optical imaging and scientific discovery. These unassuming solutions serve as the final bridge between the researcher’s work and the visualization of microscopic specimens under the lens of a microscope. Mounting media are essential substances used to preserve, protect, and enhance the quality of microscope slides, ensuring that the delicate details of specimens remain intact and accessible for analysis.
We are able to discuss the fundamental principles behind mounting media, discussing how they prevent specimen desiccation, reduce light scattering, and aid in the protection against photobleaching. Moreover, we will highlight the various considerations that researchers must keep in mind when choosing a mounting medium, including refractive index, fluorescence compatibility, and long-term stability.
- Reference for main picture: A sample preparation procedure enables acquisition of 2-channel super-resolution 3D STED image of an entire oocyte, Michaela Frolikova, Michaela Blazikova, Martin Capek, Helena Chmelova, Jan Valecka, Veronika Kolackova, Eliska Valaskova, Martin Gregor, Katerina Komrskova, Ondrej Horvath, Ivan Novotny, bioRxiv 2023.03.07.531472; doi: https://doi.org/10.1101/2023.03.07.531472
1. AD-MOUNT: ADVI mounting media
Universal Application
Choosing the appropriate mounting media is a pivotal factor in ensuring the attainment of exceptional microscopy outcomes. The utilization of mounting media serves as the conclusive phase in the comprehensive sample preparation procedure. Eager to delve deeper into this topic?
Universal Versatility and applications: Universal mounting media have been meticulously crafted to cater to a wide spectrum of fluorophores. They provide meticulously optimized safeguards against photo-bleaching and fluorescence fading, all while impeccably preserving the structural integrity and form of biological specimens, effectively counteracting the adverse effects of flattening. These media represent highly effective solutions tailored to accommodate a vast array of fluorophores.
Introducing AD-MOUNT F, Your Universal Mounting Medium
Refractive Index: 1.45
Superior Stability:
Superior Stability:
Our product guarantees steadfast fluorescence stabilization, especially with frequently employed synthetic dyes. It excels at the task of minimizing both photo-bleaching and fluorescence fading. Furthermore, it is painstakingly designed to uphold the structural integrity and natural form of biological specimens, thus effectively mitigating any undesirable flattening effects.
Introducing AD-MOUNT S, Your Go-To Solution for Superior Stability
Refractive Index: 1.47
Perfectly suited for synthetic dyes, offering impeccable stabilization. Please note that it is not advisable for use with fluorescent proteins.
Dedicated for SR microscopy
Tailored for Super-Resolution Microscopy: Custom-engineered to meet the exacting demands of high-resolution and super-resolution microscopy, featuring an elevated refractive index, extraordinary clearing properties, and unmatched stability.
Introducing AD-MOUNT C, Your Super-Resolution Specialist
Refractive Index: 1.518, with an added clearing effect
Highly endorsed for super-resolution microscopy, although it may not be compatible with expressions and SiR.
Explore our step-by-step video protocols on the VIDEO PROTOCOLS: From Washing to Mounting
Welcome to ‘From Washing to Mounting.’ This series guides you through the essential steps for preparing high-quality microscope slides, from initial washing to final mounting. Let’s get started!
2. AD-SEAL | MOUNTING SPACERS
Mounting spacers, known as AD-SEAL, play a pivotal role in microscopy, offering an array of advantages to researchers and scientists. Firstly, they serve as a reliable and straightforward solution for sealing your cover glass, ensuring the stability of your specimen during imaging and averting potential damage or movement.
Moreover, these mounting spacers serve as a protective buffer for delicate biological structures like cells and tissues. By creating a space between the cover glass and the slide, they act as a safeguard, mitigating the risk of damage from pressure or stress.
In addition to their protective role, mounting spacers contribute to enhancing the quality of your microscopy images. They establish a consistent thickness across the sample, preventing distortions or aberrations that may arise from irregularities in the slide or cover glass.
In summary, the utilization of mounting spacers in microscopy is strongly recommended due to their capacity to provide a secure and convenient sealing solution, shield fragile biological specimens, and elevate the overall quality of your microscopy images.
AD-SEAL | SQ18-10150
Inert adhesive spacers for sealing and the protection biological structures
18 mm square spacer, 10 mm inner space diameter
0.150 mm thickness
Cat. No.: ADS-18-10150-20 | 20 pcs
Cat. No.: ADS-18-10150-100 | 100 pcs
3. AD-CHAMBERS
ADVI specializes in the design and manufacturing of chambers and sample holders tailored for delicate or sizable specimens. These components play a crucial role in clearing techniques, and as such, they are meticulously engineered to be both highly functional and chemically inert. Crafted from premium-quality stainless steel, they feature precision surface grinding to ensure optimal performance.
Our products boast a versatile range of applications, thanks to their high-quality stainless steel construction with a resilient polished surface. They are well-suited for accommodating a diverse array of samples and are compatible with a wide range of clearing chemicals.
FIX OR CUSTOM
Fixed size: 10x10mm, chamber thickness 3 mm; 1pc, 5pc
Fixed size: 15x15mm, chamber thickness 6 mm; 1pc, 5pc
Customized thickness: 10x10mm, chamber customized thickness1.5 – 3 mm; 1pc, 5pc
Customized thickness: 15x15mm, chamber customized thickness 3 – 6 mm; 1pc, 5pc
Fully customized: chamber size customized, chamber thickness customized; 1pc, 5pc
Are you interested?
#science #fluorescencemicroscopy #microscopy #mountingmedia #lightmicroscopy #confocal
ADVI produces high-quality of mounting media – Developed in collaboration with Institute of molecular genetics of Czech academy of sciences.
Looking for a customized solution for your specific needs? Our team of experts are here to provide you with a solution that is a perfect fit for your needs.
Contact us today to discuss your project and discover how our customised solutions can maximise your potential for success.